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Identification of the molecular components of haemopoietic lineage specificity
Reference
9708836
Principal Investigator / Supervisor
Dr Gerard Brady
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
92,173
Status
Completed
Type
Research Grant
Start date
01/01/1998
End date
01/06/2000
Duration
30 months
Abstract
Two approaches will be taken to determine the macrophage-specificity of macrophage-colony stimulating factor (M-CSF). In the first approach, recombinant constructs of the M-CSF receptor (c-fms) will be prepared in which domains of c-fms are swapped with the corresponding domains from closely related receptors which are unable to support macrophage differentiation. The ability of the chimeric receptors to support macrophage growth will be established by transfection in embryonic stem cells capable of haemopoietic differentiation. In the second approach, the yeast two-hybrid system will be used to identify proteins which interact only with c-fms and not with closely related receptors.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1997 (ROPA1997) [1997]
Funding Scheme
X – not Funded via a specific Funding Scheme
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