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Gal transferase induced ablation of undifferentiated human ES cells

Reference0014935
Principal Investigator / Supervisor Dr James McWhir
Co-Investigators /
Co-Supervisors
Institution The Roslin Institute
DepartmentGene Expression and Development
Funding typeResearch
Value (£) 119,712
StatusCompleted
TypeResearch Grant
Start date 02/07/2001
End date 03/10/2003
Duration27 months

Abstract

Transplantation therapy using ES-derived cell types following in vitro differentiation is accompanied by a risk of contamination with undifferentiated and potentially tumorigenic ES cells. The objective of this project is to modify human ES cells such that undifferentiated cells express a xenotransplantation epitope (1,3 gal) which renders them sensitive to complement mediated lysis in vivo. We will also investigate the feasibility of rendering the expression of the catalytic subunit of the telomerase enzyme (hTERT) inducible in culture as a means of prolonging the proliferative life span of differentiated cells.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative ROPA 2000 (ROPA2000) [2000]
Funding SchemeX – not Funded via a specific Funding Scheme
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