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Award details
Development of a novel transgenic approach to the study of clock gene regulation in a seasonally breeding mammal
Reference
0014904
Principal Investigator / Supervisor
Professor Andrew Loudon
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
172,772
Status
Completed
Type
Research Grant
Start date
06/05/2001
End date
28/08/2003
Duration
28 months
Abstract
This project aims to develop a transgenic technology in a novel rodent species, the Syrian hamster, taking transgenic technology to important physiological systems, which cannot be studied in laboratory rodents. The transgene will report dynamic circadian transcriptional profiles of the circadian clock gene per1 created by direct pro-nuclear injection of DNA constructs, and use of a novel destabilised green fluorescent reporter protein. The transgenic animal will permit real-time imaging and characterisation of clock-gene involvement in the seasonal neurobiological response to melatonin and photoperiod in vitro and also define the impact of a circadian clock gene mutation on circadian cells and tissues.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 2000 (ROPA2000) [2000]
Funding Scheme
X – not Funded via a specific Funding Scheme
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