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Directed evolution of proteinase inhibitors
Reference
0014846
Principal Investigator / Supervisor
Professor John Kay
Co-Investigators /
Co-Supervisors
Institution
Cardiff University
Department
School of Biosciences
Funding type
Research
Value (£)
78,316
Status
Completed
Type
Research Grant
Start date
12/11/2001
End date
12/01/2004
Duration
26 months
Abstract
Our recent work has revealed an unprecedented mode of enzyme inhibition in which a 33-residue polypeptide potently inhibits its target proteinase and no other. This novel mechanism will be exploited to generate inhibitors of other proteinases. Phage display libraries will be i) constructed to contain a random collection of variant 99 bp sequences generated by error-prone PCR and ii) assessed by biopanning for their ability to bind to a new target proteinase which, for proof-of-concept purposes, will be plasmepsin II from the malaria parasite, P. falciparum. The phage libraries will also be a valuable resource for eventual exploitation to identify inhibitors of an unlimited number of proteinases.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 2000 (ROPA2000) [2000]
Funding Scheme
X – not Funded via a specific Funding Scheme
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